공용장비

(교내)자산관리번호 : 032104-0000-00 (ZEUS)시설장비등록번호 : NFEC-2011-02-140375

공용장비 소개

공용장비 설명: 1. Ultimate range of fluorophore excitation and emission from 450-730nm.
2. Up to five different reporter fluorphores can be multiplexed per each sample tube and FRET application were available.
3. The availability of 12 uniform-temperature wells at each temperature within the gradient enables evaluation and optimization of the maximum number of reaction components and parameters in a single experiment.
4. 96 sample tubes are sequentially illuminated by 6 filtered light-emitting diodes(LEDs). All 96 samples are independently excited and detected for each color to minimize cross-talk.
5. It is possible for real-time PCR experiment in stand-alone mode without computer system.
6. Sample data can be reanalyzed at any time; raw data files are user accessible.
7. It is possible for exchanging 384 block reaction module for high-throughput system.
8. Precision Melt Analysis software can import and analyze data files generated from the 96 block or 384 block to genotype samples based on the thermal denaturing profiles of double-stranded DNA.
9. Base thermal cycler offers precise thermal control and a temperature gradient feature.
10. Up to 4 units can be operated with one PC system simultaneously.
11. Protocol auto-writer function of thermal cycler generates an optimal protocol based on your polymerase primers and product length.
12. NIST traceable temperature performance.

장비구성 및 성능: Gradient thermal cycler 1 set
96 block continuous fluorescence detector with fast reaction module 1 set
Real time PCR software 1 copy

장비활용 예시: Real-time PCR(Polymerase chain reaction) 은 thermal cycler와 분광 형광 광도계가 일체화된 장치로 다양한 형광물질을 이용하여 실시간으로 PCR 증폭산물의 생성과정을 모니터링하여 target DNA의 양을 분석하는 장비로 다양한 분야에 응용됨. Real-time PCR은 증폭 산물 확인을 위한 전기영동이 필요 없으며 증폭이 지수함수적으로 일어나는 영역에서 증폭 산물량을 비교하여 보다 정확한 정량이 가능한 신속성과 정량성이 뛰어난 방법임.